A heat-stable DNA polymerase is used in the reaction process. c. It is associated with a sliding clamp only on the leading strand. C. A new DNA strand is synthesized with the same polarity (5' and 3' ends) as the template strand. Explanation: DNA polymerase is an enzyme which is used to make new DNA. c. Is created from embryonic stem cells. B. * 20. Question: Question 20 Which Of The Following Statements Is Not True? The Lagging Strand Of DNA Is Started By An RNA Primer. d. Generating an organism that is a genetic copy of another organism by bypassing sexual reproduce. DNA replication proceeds in only one direction around the bacterial chromosome. B. DNA replication produces a DNA duplex that consists of one new strand of DNA and one old strand of DNA. Privacy Answer. 3. A. DNA Polymerase Joins Nucleotides In One Direction (5' To 3') Only. A. PCR requires DNA primers to initiate synthesis of a strand complimentary in sequence to the template. The term "cloning" refers to: a. a. DNA Polymerase b. dNTPs c. Helicase d. Primers e. None of the above (all are required) Which IS NOT true about complimentary base-pairing: a. a. DNA Polymerase b. dNTPs c. Helicase d. Primers e. None of the above (all are required) Which IS NOT true about complimentary base-pairing: a. replication, DNA polymerase adds nucleotides to form a new DNA b. d. Generating an organism that is a genetic copy of another organism by bypassing sexual reproduce. Which of the following enzyme is suitable for dephosphorylation at 5’ end of a DNA strand? Answer: C Topic: Polymerase Chain Reaction Section: 25.8 Difficulty Level: Medium 83) The thermal cycler heats the DNA sample to: a) 90 o C; then cools down to: b) 50-60 o C; and after it is warmed to c) about 70 o C. This steps are done in order to a) a) denature the DNA sample; b) allow the primers to bind; c) denature the DNA again. C) Replication of DNA occurs immediately after cytokinesis as the cell prepares to form new daughter cells. It can act as an exonuclease to cut single-stranded DNA Taq polymerase It lacks a 3 to 5 exonuclease proofreading activity. Question: A 28-year old bride-to-be is getting married the first week of Terms She wants to wear …. b. (a) Primers, dNTPs and DNA polymerase (b) DNA, CaCl 2 and nuclease (c) Mg + 2, DNA (d) Both (a) and (c) Answer: (d) Both (a) and (c) Question 6. 22. Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase. Show transcribed image text Which of the following IS NOT required for PCR? Is defined as a mix of human and animal DNA. nucleotides. b. Which of the following is required to perform polymerase chain reaction ? The DNA polymerase can not replicate DNA de novo, it required a free 3’ OH group to do so which is provided by the primer. on each plate to each other? O It must polymerize DNA 5' to 3'. The terms and conditions highlight that the company accepts no responsibility to third parties for unauthorized use of its products.