I am trying to calculate the phenol amount using gallic acid as standard and i try to express the amount of phenols as mg GAE (Gallic Acid Equivalents) / gram sample. The medium was removed and cells were washed with PBS before treating with the test sample (500 μg/mL) diluted in the medium containing 25 μM DCFH-DA for 1 hr. Sie können Ihre Einstellungen jederzeit ändern. In general, the study shows that the plants grown in the aeroponic system had a higher yield and comparable phenolics, flavonoids, and antioxidant properties as compared to those grown in the soil. of gallic/tannic acid established from calibration curve in mg/ml; V=the volume of the extract in ml and N=the wt of the plant extract in gm]. (1989) [41] have reported a greater dry-matter accumulation in maize (Zea mays L.) on the hydroponic system than in well-fertilized, irrigated sandy-loam soil when planting pattern and density were the same. 0 0. shimp. I have read many papers but am unable to understand the calculations how to express total phenolic contents as mg GAE/ gram of dry weight. The differences in phenolic content, however, were not found to be statistically significant for all the leafy vegetables (basil, LSD = 32.50, ; chard, LSD = 41.15, ; parsley, LSD = 18.00, ; red kale, LSD = 22.79, ) while grown in two types of cultivation systems. So i want to know the concentration in mg GAE/g sample. (2010) [42] while grown in hydroponics as compared to those grown in soil. � Production and hosting by Elsevier Ltd on behalf of Taibah University. Briefly, 0.2 mL of the diluted sample extract was transferred in tubes containing 1.0 mL of a 1/10 … 109 0 obj <>stream ***final concentration = (0.342+0.0307)/11.924=0.031256 mg/mL, ***initial concentration= 0.031256*10=0.31256 mg/mL, ***total mass in your extract = initial concentration*volume = 0.31256*15=4.68844mg, ***the content is: mass of phenolic /mass of sample:4.68844 mg / 0.1 g (100 mg). Given the following: weight of sample = 0.1 g; Absorbance at 760 nm = 0.342 All determinations were carried out in triplicate. Reaction with Folin-Ciocalteu solution: - I add 0.5 ml of my clear extract in a 50 ml flask. The antioxidant capacity of fruits and vegetables can be tested using a wide variety of methods. This spectral and fluorescence study of the drug will be helpful for confirming the identity and purity of the genuine drug. B. Pridham, “The relative antioxidant activities of plant-derived polyphenolic flavonoids,”, N. Sugihara, T. Arakawa, M. Ohnishi, and K. Furuno, “Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with, C. A. Rice-Evans, N. J. Miller, and G. Paganga, “Structure-antioxidant activity relationships of flavonoids and phenolic acids,”, N. Ramarathnam, H. Ochi, and M. Takeuchi, “Antioxidant defense system in vegetable extracts,” in, H. Tapiero, K. D. Tew, G. N. Ba, and G. Mathé, “Polyphenols: do they play a role in the prevention of human pathologies?”, M. J. Laughton, P. J. Evans, M. A. Moroney, J. R. S. Hoult, and B. Halliwell, “Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives. This research was partially funded by the United States Department of Agriculture, Agricultural Research Service (USDA-ARS), Specific Cooperative Agreement no. Here's the question, have I done it right with this calculation 21 * 0.005L/ 0.05 g = 2.1 mg GAE/g extract? endstream endobj startxref Prepare serial dilutions of Trolox standards: standard (μL)/H2O/Ethanol (μL) 20/980, 40/960, 100/900 200/800. I've maserated 200g of fresh pomelo with 200 mL ethanol, then I've got my crude extract from rotary evaporation process. The interest in phenolic compounds derived from vegetables and their roles in nutrition are therefore increasing [32, 37]. In ... methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. The dried extract was used for further analysis. The hydroxyl groups in plant extracts are responsible for facilitating free. Tha means that I have to include also this dilution factor for my standard curve? How to build Gallic Acid Curve and calculate Total Phenolic Content? The total product yield was, however, higher in aeroponically grown crops. (Refer to attached material). Similar to phenolic content leafy vegetables had higher flavonoid content compared to fruit crops. © 2008-2020 ResearchGate GmbH. Please show me the step-by-step calculation of total phenolic content determination. What does a serving constitute?”, M. P. Kähkönen, A. I. Hopia, H. J. Vuorela et al., “Antioxidant activity of plant extracts containing phenolic compounds,”, C. A. Rice-Evans, N. J. Miller, P. G. Bolwell, P. M. Bramley, and J. Copyright © 2020 Elsevier B.V. or its licensors or contributors. © 2016 The Authors. The presence of a high total phenolic content shows that the flowers of N. bracteata Benth may possess antioxidant properties, which could lead to a new field of research in the future. This study quantitatively determined the total phenolic contents in ethanolic and aqueous extracts of Gul-e-Zoofa (flowers of Nepeta bracteata Benth) using a spectrophotometric method. I filtered the solvent and do use 1ml of the extract for analysis. The absorbance was measured at 515 nm. The total phenolic content (TPC) was determined by spectrophotometry, using gallic acid as a standard, according the method described by Singleton and Rossi (1965). I also did kinda the same with my samples: BUT I solved 2.0 gram of sample in 10 mL of (95%) MeOH. Gallic acid showed 95% radical scavenging activity at 20 μM. Yes Muhammad that's how you do it. Indoor cultivation carried out under the control and optimized environmental conditions can further maximize the yield of the product and also eliminate the problems linked to fluctuations in the outdoor weather conditions. The total phenolic contents of the alcoholic and aqueous extracts were found to be 326.28 and 319.14 mg/g of the Gallic acid equivalent (GAE), respectively. 66 0 obj <> endobj The total phenolics of the extracts were determined using the Folin and Ciocalteu reagent, following the method described by Singleton and Rossi [20] with slight modifications. I have to find out the total phenolic contents in five different plant extracts. The fluorescence characteristics of the powdered drug were also observed. Relationship to antioxidant activity and to iron ion-reducing ability,”, P. Cos, L. Ying, M. Calomme et al., “Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers,”, A. Wojdyło, J. Oszmiański, and R. Czemerys, “Antioxidant activity and phenolic compounds in 32 selected herbs,”, M. H. Miller, G. K. Walker, M. Tollenaar, and K. G. Alexander, “Growth and yield of maize (, C. Sgherri, S. Cecconami, C. Pinzino, F. Navari-Izzo, and R. Izzo, “Levels of antioxidants and nutraceuticals in basil grown in hydroponics and soil,”, N. Deepa, C. Kaur, B. Singh, and H. C. Kapoor, “Antioxidant activity in some red sweet pepper cultivars,”, U. Imeh and S. Khokhar, “Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations,”, B. Ou, D. Huang, M. Hampsch-Woodill, J. The mixture was allowed to stand for 6 min, then 150 μL of aluminium trichloride (10%) was added and Antioxidant properties of AG and FG crops were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant (CAA) assays. h�b```f`` endstream endobj startxref Hope this little explanation is helpful. The test sample (0.2 mL) was mixed with 0.6 mL of water and 0.2 mL of Folin-Ciocalteu’s phenol reagent (1 : 1). The cellular antioxidant activity was measured in HepG2 cells as described by Wolfe and Rui [26]. By continuing you agree to the use of cookies. f˃H�~ɢ VYy&�� �Vc"��@d���p��H2L���A���4�����ր�30Ҝ�����{� �+f